고농도 포도당 환경에서 EMD(Enamel Matrix Derivatives)가 인간 치주인대 세포와 뼈모세포양 세포(MC3T3-E1)에 미치는 영향

이백수; 권용대; 김선욱; 주성숙

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고농도 포도당 환경에서 EMD(Enamel Matrix Derivatives)가 인간 치주인대 세포와 뼈모세포양 세포(MC3T3-E1)에 미치는 영향
EFFECT OF EMD ON HUMAN PERIODONTAL LIGAMENT-DERIVED CELLS AND OSTEOBLAST-LIKE CELLS (MC3T3-E1) IN HIGH GLUCOSE CONDITION

대한구강악안면외과학회지 2008년 34권 5호 p.532 ~ 536

이백수, 권용대, 김선욱, 주성숙,

소속 상세정보

이백수 ( Lee Baek-Soo ) - 경희대학교 치과대학 구강악안면외과학교실
권용대 ( Kwon Yong-Dae ) - 경희대학교 치의학전문대학원 구강악안면외과학교실
김선욱 ( Kim Sun-Wook ) - 경희대학교 치과대학 구강악안면외과학교실
주성숙 ( Jue Sung-Sook ) - 경희대학교 치과대학 구강생물학연구소

KMID : 0355620080340050532

Abstract

Purpose: This study was designed to evaluate effect of EMD on proliferation of HPDLCs and MC3T3-E1 cells in high glucose condition in vitro.

Material and method: The Human PDL fibroblasts(HPDLCs) were obtained through typical way and the cells used in this experiment were divided in 4 groups. 1x104/ml HPDLCs suspension was cultured in typical DMEM and assigned to group 1. The cells cultured in DMEM which included 400mg/dl glucose are allocated to group 3. Group 2 and 4 are established by adding EMD to group 1 and 3 respectively. These control and experimental groups had been cultured for 24 and 48 hours, and MTT assay was conducted. The differences of each group in cellular proliferation was evaluated. The same experiment was conducted for preosteoblast (MC3T3-E1) with adding 25㎍/ml EMD.

Results: EMD had the same effect on both PDL cells and MCT3T3-E1 cells. The experimental group had more meaningful differences and active cellular proliferation than the control group did. The EMD accelerated cellular proliferation not only in normal glucose condition but also in high glucose condition. The same results were observed via MTT assay; EMD-added experimental group had more meaningful differences and showed higher cellular activity than control group did. Each experimental and control group was inspected for statistical significance through Kruskal-Wallis Test. Statistical significances were observed among these groups. (SPSS 12.0 Chicago, IL, USA, p=0.008, p=0.011)

Conclusion: EMD is considered to accelerate proliferation of PDL cells and MC3T3-E1 cells in high glucose condition as well as normal glucose condition.